Cannabis Extracts and Their Cytotoxic Effects on Human Erythrocytes, Fibroblasts, and Murine Melanoma
Please use this link to access this publication.
Abstract
Most of the pharmacological effects of Cannabis species have been attributed to their cannabinoid constituents, primarily Δ9-tetrahydrocannabinol and cannabidiol. Efficient methods of extraction, purification, and characterization of these compounds are necessary to obtain both enriched preparations and isolated compounds for therapeutic applications. In this study, forensic samples of Cannabis sativa L., Cannabaceae, were subjected to extraction, using maceration in two different solvents, methanol, and diethyl ether. These extracts were subjected to thermal decarboxylation and purification by liquid–liquid fractionation. Δ9-Tetrahydrocannabinol and cannabidiol concentrations from various extracts were quantified by high-performance liquid chromatography with UV detection. Extract cytotoxicity was assayed in vitro using human erythrocytes, mouse embryonic fibroblasts (NIH3T3), and murine melanoma (B16F10) cells. The B16F10 cell death mechanism was also evaluated after treatment with extract samples. The results demonstrated promising effects of various cannabinoid extracts in a murine melanoma cancer line.
This library aims to empower you with knowledge but it does not replace the personalized advice and guidance a healthcare professional can provide. Before implementing any changes to your health regimen based on the contents of this library, we strongly advise you to consult with a qualified healthcare professional. Your doctor’s expertise is essential for interpreting how these insights may apply to your unique health circumstances.