Extraction of cannabinoids from cannabis sativa L plant and its potential antimicrobial activity’
Abstract
Drug resistance of microorganisms is a big threat for the human health. In order to overcome this problem an alternative potential treatment is the use of herbal medicines. Plants are not only considered as dietery supplements to living organisms but also tradionally used for treating many diseases and no sign of drug resistance has been reported till now. The plant leaves were identified as Cannabis sativa L. The cannabinoids were extracted by aqueous extraction found a total yield of 3.8g while as acetone extract 4.8g. The protein content in crude extract of Cannabis sativa L. for aqueous extract was found 112µg/ml and for acetone extracts 160µg/ml. The molecular weight of protein by SDS PGAGE was found to be 70KDa. The HPLC intension percentage for aqueous extract was 11 while for acetone extract it was found 25. The acetone extract exhibited higher antimicrobial activity by showing zone of inhibition against bacteria Pseudomona aeruginosa, Vibro cholerae and fungi Cryptococcus neoforms, Candida albicans . The cannabiniods extracts showed the antioxidant activity by changing to yellow colour.
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